Table 2 Primer sequences of real-time quantitative polymerase chain reaction (RT-qPCR)
Genes | Sequences (5ʹ-3ʹ) |
|---|---|
CACNA1C | F: TCCTCCGCTCTGCCTCACTAG |
R: CACTGCCAATGCCTGATGATGAAC | |
CACNA2D1 | F: ACTGCTGCTGCCTGGTCTATTC |
R: ATCCTCCATCTCAACTGCCTCAAG | |
CACNB2 | F: CGCTCCTATCCGTTCTGCTTCC |
R: TCCTGGGTTTCCGAGTCAAATGTC | |
α-SMA | F: CTCTGGACGCACAACTGGCAC |
R: CACGCTCAGCAGTAGTAACGAAGG | |
Col1A1 | F: AAAGATGGACTCAACGGTCTC |
R: CATCGTGAGCCTTCTCTTGAG | |
Col1A2 | F: CTCCATGGTGAGTTTGGTCTC |
R: CTTCCAATAGGACCAGTAGGAC | |
GAPDH | F: TTGCCATCAATGACCCCTT |
R: CGCCCCACTTGATTTTGGA |