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Fig. 8 | Eye and Vision

Fig. 8

From: An L-type calcium channel blocker nimodipine exerts anti-fibrotic effects by attenuating TGF-β1 induced calcium response in an in vitro model of thyroid eye disease

Fig. 8

An activation of STAT1 is involved in fibrogenesis during TED. a, b Representative IHC staining for p-STAT1 (Ser727) (a) and total STAT1 (b) were performed on paraffin-embedded biopsy sections. Scale bar, 100 μm. c, d Analysis of IHC scoring of p-STAT1 (Ser727) (c) and total STAT1 (d), n = 5. e, f The representative data and statistical analysis of p-STAT1 (Ser727) levels of TED-OFs and HC-OFs detected by flow cytometry, n = 4. g, h WB confirmed up-regulated expression of p-STAT1(Ser727) in response to TGF-β1 stimulation in TED-OFs, n = 3. **P < 0.01, ***P < 0.001, unpaired Student’s t-test. STAT1, signal transducer and activator of transcription 1; TED, thyroid eye disease; HC, healthy control; IHC, immunohistochemistry, TED-OFs, OFs derived from patients with TED; HC-OFs, OFs derived from HC donors; WB, western blot analysis; TGF-β1, transforming growth factor-beta 1; FMO, fluorescence minus one; GAPDH, glyceraldehyde-3-phosphate dehydrogenase

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Eye and Vision

ISSN: 2326-0254