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Fig. 6 | Eye and Vision

Fig. 6

From: An L-type calcium channel blocker nimodipine exerts anti-fibrotic effects by attenuating TGF-β1 induced calcium response in an in vitro model of thyroid eye disease

Fig. 6

An aberrant CaMKII activation is involved in fibrogenesis during TED. a, b Representative IHC staining for p-CaMKII (Thr286/287) (a) and total CaMKII (b) were performed on paraffin-embedded biopsy sections obtained from orbital adipose connective tissues of patients with TED and HC donors. Scale bar, 100 μm. c, d Analysis of IHC scoring for p-CaMKII (Thr286/287) (c) and total CaMKII (d), n = 6. e, f WB showed up-regulated expression of p-CaMKII (Thr286/287) in the orbital adipose connective tissues of patients with TED compared to HC donors, n = 4. g, h WB showed up-regulated expression of p-CaMKII (Thr286/287) in response to TGF-β1 stimulation in TED-OFs, n = 3. The significance was determined by unpaired Student’s t-test (c, d, f) or one-way ANOVA (h). **P < 0.01; ***P < 0.001; ns, not significant. CaMKII, Ca2+/calmodulin-dependent protein kinase II; TED, thyroid eye disease; HC, healthy control; IHC, immunohistochemistry; WB, western blot analysis; TGF-β1, transforming growth factor-beta 1; TED-OFs, OFs derived from patients with TED; GAPDH, glyceraldehyde-3-phosphate dehydrogenase

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