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Fig. 4 | Eye and Vision

Fig. 4

From: An L-type calcium channel blocker nimodipine exerts anti-fibrotic effects by attenuating TGF-β1 induced calcium response in an in vitro model of thyroid eye disease

Fig. 4

Nimodipine alleviated TGF-β1 induced cell proliferation of OFs. a, b The representative images and statistical analysis of EdU-positive TED-OFs in different groups are shown. Before EdU assay, OFs were pretreated with 0 (control), 20, 40 or 60 μmol/L nimodipine for 5 min, followed by 10 ng/mL TGF-β1 stimulation for 24 h. One-way ANOVA, n = 4; *P < 0.05; **P < 0.01; ****P < 0.0001. TGF-β1, transforming growth factor-beta 1; OF, orbital fibroblast; TED-OFs, OFs derived from patients with thyroid eye disease; EdU, 5-ethynyl-2′-deoxyuridine; Nimo, nimodipine. Scale bar, 100 μm

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Eye and Vision

ISSN: 2326-0254