Fig. 3
From: Homocysteine and mitochondrial quality control in diabetic retinopathy
Glucose-homocysteine and mitophagy in retinal endothelial cells. Human retinal endothelial cells were analyzed for mitophagosome formation by (a) staining with an autophagy dye and visualizing with an Apotome using 20 × objective (scale bar = 20 μm). b Arithmetic mean intensity (AMI) of green fluorescence from three different cell preparations was plotted, and each measurement was made in triplicates. Green = mitophagosomes, blue = nucleus stained with Hoechst and red = mitochondria stained with MitoTracker red (MTR); NG and HG, 5 mM and 20 mM d-glucose, respectively; Hcy and Hcy/GY, 20 mM d-glucose with homocysteine, without and with GYY4137, respectively; l-Gl, 20 mM l-glucose; Rapa, 5 mM d-glucose with Rapamycin. *P < 0.05 vs. NG; #P < 0.05 vs. HG